New experimental design: Dehydration induced uremia and oxidative stress on male albino rats, innovative approach to researchers for further study on kidney disease

Abstract

Uremia means excess nitrogenous waste products in the blood and their toxic effects. Dehydration or water deprivation of body that decreases urine excretion allows urea and other protein waste products to accumulate and oxidative stress develops. The aims of this present study are to investigate uremia & oxidative stress are caused by dehydration and this study is the introductory experiment for further research of “Protection of dehydration induced uremia and oxidative stress by some selective medicinal plant extract” and a new methods introduction to dehydration. In this context, this experimental studies set up dehydration induce uremia and oxidative stress on albino rats in five groups-one control-normal food and water provided, and four dehydrating groups based on duration dependent of water providing and limited water providing. After 45 days of experiment we have observed body weight, super-oxide dismutase (SOD) and catalase activity lower and blood urea, blood creatinine and Malondialdehyde (MDA) in dehydrating groups are higher than control group. We have concluded that those rats received little amount of water than other dehydrating animals and dehydration period was long than others, these rats had been achieved more dehydration by significantly lower its body weight, catalase & SOD enzyme in plasma & higher in blood urea level, blood creatinine level & lipidperoxidation (MDA activity) than other three dehydrating groups. So, severe uremia and oxidative stress occurred in dehydrating animals.