Biological Impact of Feeding Rats with Cry Protein Based Diet

Abstract

Effect of Bt cry protein depends upon the concentration and length of exposure. The current study suggest that Bt cry protein ingestion induces a time dependent decreases in food intake capacity in treated rats through there is an increase in body weight. This work was conducted on the study of thebiological impact study on albino rats, with a range of combined parameters including Total protein & DNA fragmentatin to evaluate the impact of a cry protein based diet on animal health. The procedures were performed in the liver, kidney, and spleen, stomach tissues. The percentage of survival rate thus calculated, exhibited 100%survivability in both groups. Experimental researches in mice showed that DNA can persist in fragmented form in the gastrointestinal tract, penetrate the intestinal wall and reach the nuclei of leukocytes, spleen, liver cells. We studied the percentage of DNA fragmentation of isolated tissue (such liver, spleen, kidney stomach & blood) by DPA assay method and taking absorbance at 600nm by colorimetric method. It was observed that percentage of DNA fragmentation are higher in Bt cry protein treated rats as compared to control for stomach, kidney, liver tissue whereas lower value was obtained in spleen and blood samples. In addition, oxidative stress induction was observed in the stomach, kidney, liver after 90 days of infection initiation. The elevated DNA fragmentation may be related to increase oxidative stress.